Measuring Performance of an Automated and Miniaturized LANCE Ultra cAMP Assay for the Gi-coupled 5-HT1A Receptor– a Comparative Study
نویسندگان
چکیده
The LANCE® Ultra cAMP assay is a secondgeneration LANCE time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated guanosine triphosphate binding protein-coupled receptors (GPCRs). The homogeneous two-component assay is based on the competition between europium chelate (Eu)-labeled cAMP and cellular cAMP for binding to high-affinity anti-cAMP monoclonal antibodies labeled with the ULightTM dye. The assay principle is shown in Figure 1.
منابع مشابه
LANCE Ultra cAMP: A New TR-FRET cAMP Assay for Gs- and Gi-Coupled Receptors
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